Stem Cell Applications | Corning

Look to Corning CoolCell alcohol-free freezing containers, cryogenic vials, and accessories for reproducible, controlled-rate freezing for stem cells, primary cells, cell lines, and more.

Complete and ready-to-use, NutriStem provides a simplified medium for consistent performance, as well as increased reproducibility in hPSC long-term culture. 

The extracellular matrix (ECM) is a complex material that serves to stabilize tissues, promote cell attachment, and modulate cell functionality by specifically interacting with cell surface receptors. This material has been shown to play structural, biochemical and mechanical roles in the normal growth and differentiation of various cell types. Corning Matrigel Matrix, an ECM-based material isolated from EHS mouse tumors, is a widely used solubilized basement membrane preparation. Based on its physiological composition and functionality, Matrigel Matrix effectively models the physical interplay between diverse cell types and the ECM microenvironment in-vivo.

Expansion of stem cells and some primary cells requires either animal-origin components in culture media or coating of the culture vessels with human or animal-derived extracellular matrix (ECM) protein. Growing concerns about introducing human and animal-derived pathogens into the culture necessitate the need for animal- free (xeno-free and human-origin components-free) culture environment. Herein, we present defined and synthetic surfaces for cell culture. Corning® PureCoat™ ECM Mimetic cultureware collagen I peptide and fibronectin peptide support culture of cell types requiring collagen I or fibronectin protein-coated surfaces. Corning Synthemax® surface consists of a vitronectin peptide and supports undifferentiated expansion of human pluripotent stem cells (hPSC) and neural progenitor cells (NPCs). ECM Mimetic and Synthemax cultureware are pre-coated, synthetic, animal-free, and room temperature stable. Synthemax-SC is available for self-coating.

We demonstrate expansion and functionality of several clinically relevant cell types including hPSCs, mesenchymal stem cells (MSCs), human keratinocytes (HKN), endothelial colony forming cells (ECFCs), NPCs and cell lines for biomanufacturing (CHO and Vero) on these surfaces. Corning Synthemax and ECM Mimetic cultureware are versatile surfaces compatible with multiple media for culture of various cell types providing a ready to use alternative to ECM protein coating where animal-free and defined conditions are desirable.