Banner image credit: MDI Laboratory, ME
Explore Organoid Model Environments
Explore Organoid Model Environments
Organoids are generated from both pluripotent stem cells (PSCs) and adult stem cells (ASCs). Self-renewal and differentiation of stem cells is influenced by growth factors and extracellular matrices (ECM) that provide the required scaffold to support cell attachment and growth during organoid formation. Hydrogels such as Corning Matrigel® Matrix and Corning® Collagen are popular scaffold choices to support cell expansion in organoid cultures.
Stem cells and/or organ progenitors from normal or diseased tissue can be mixed with Matrigel matrix or Collagen to create mini-organs of the kidney, thyroid, liver, brain, lung, intestine, prostate, and pancreas. Organoids support advancements in the study of organogenesis, disease modeling, and subsequent patient-specific therapies. For example, the combination of genome editing using CRISPR-Cas9 and organoid cultures allows researchers to evaluate DNA repair of patient-specific mutations found in certain cancers and perform genetic screens. Hydrogels such as Collagen and Matrigel Matrix can be used as bio-inks to allow precise positioning and embedding of living cells/organoids during 3D bioprinting. Organoids are also being used as physiologically relevant models for the development of new therapeutic drug candidates.
TYPES
Organoid Types
Publications
Supporting Publications for Organoid Culture
Supporting Publications for Organoid Culture
Cancer Phenotypes
Discover research on organoid cultures for the analysis of cancer phenotypes.
Engineering Stem Cell Organoids
Learn more about basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be implemented.
Disease Modeling
Discover research examining disease modeling in stem-cell derived 3D organoid systems.
Organoids and Epithelial Translational Medicine
Learn more about the advantages of stem cell-derived organoid models over existing culture systems, as well as recent advances in epithelial tissue-specific organoids.
Testimonials
Customer Success Stories
Customer Success Stories
"Matrigel Matrix seems to have a lot of excellent growth factors in there." - Colin Bishop, Ph.D., Professor, Wake Forest Baptist Health
Read more about how Corning Matrigel matrix is helping to make 3D cell culture easier than ever at Wake Forest Baptist Health.
"We can recapitulate the same biological behavior and histopathological features..." - Amanda Linkous Ph.D.
Learn more about how Amanda Linkous, Ph.D. cultured and differentiated cerebral organoids in Corning Matrigel Matrix to mimic biological behavior and histopathological features of patient glioblastomas.
Featured Resources
Application Support for Organoid Models
Solutions
Solutions for Organoid Models
Natural Hydrogels
Natural Hydrogels
Learn MoreNatural hydrogels, such as Matrigel matrix and Corning Collagen Type I, are formed from ECM proteins and can be beneficial for supporting cell viability, cell migration, function, and differentiation. Matrigel matrix mimics the mechanical and chemical properties of the in vivo ECM and provides signaling cues via basement membrane ligands involved in cell attachment and survival. It is ideal for use in organoid, cancer and stem cell applications.
Collagen is the main structural component of the extracellular matrix. Corning Collagen Type I supports in vivo-like 3D growth and differentiation and can be used in the study of cell invasion, cell sensitivity to anti-cancer drugs, cell proliferation, and cell migration.
Natural hydrogels, such as Matrigel matrix and Corning Collagen Type I, are formed from ECM proteins and can be beneficial for supporting cell viability, cell migration, function, and differentiation. Matrigel matrix mimics the mechanical and chemical properties of the in vivo ECM and provides signaling cues via basement membrane ligands involved in cell attachment and survival. It is ideal for use in organoid, cancer and stem cell applications.
Collagen is the main structural component of the extracellular matrix. Corning Collagen Type I supports in vivo-like 3D growth and differentiation and can be used in the study of cell invasion, cell sensitivity to anti-cancer drugs, cell proliferation, and cell migration.
Synthetic Hydrogels
Synthetic Hydrogels
Learn MoreIn cases where bioactive compounds such as growth factors can potentially interfere with the specific cell behaviors or responses, synthetic hydrogels can be a good choice for 3D cell culture applications. Corning PuraMatrix™ Peptide Hydrogel forms a 3D environment for cells that can be plated on top of the hydrogel to study cell movement or suspended within the matrix for 3D encapsulation applications.
In cases where bioactive compounds such as growth factors can potentially interfere with the specific cell behaviors or responses, synthetic hydrogels can be a good choice for 3D cell culture applications. Corning PuraMatrix™ Peptide Hydrogel forms a 3D environment for cells that can be plated on top of the hydrogel to study cell movement or suspended within the matrix for 3D encapsulation applications.
Cell Recovery Solutions
Cell Recovery Solutions
Learn MoreCorning Cell Recovery Solution is used to recover cells and organoids from Corning Matrigel Matrix by depolymerizing thick layer of gelled Matrigel Matrix.
Corning Cell Recovery Solution is used to recover cells and organoids from Corning Matrigel Matrix by depolymerizing thick layer of gelled Matrigel Matrix.
Permeable Supports
Permeable Supports
Learn MorePermeable supports are particularly well suited for growing organotypic models as their design makes it possible to bathe cells both apically and basolaterally or grow epithelial cell models at the air-liquid interface (ALI).
Permeable supports are particularly well suited for growing organotypic models as their design makes it possible to bathe cells both apically and basolaterally or grow epithelial cell models at the air-liquid interface (ALI).
Other Surfaces
Other Surfaces
Learn MoreHighly consistent ECM formulation that enables the study of 3D cell differentiation and functionality. It is the major component of Matrigel Matrix.
Ultra-Low Attachment (Spheroids)
Ultra-Low Attachment (ULA) surfaces feature a covalently bound hydrogel layer that effectively inhibits cellular attachment. It forces cells into a suspended states that enables 3D cell culture
Highly consistent ECM formulation that enables the study of 3D cell differentiation and functionality. It is the major component of Matrigel Matrix.
Ultra-Low Attachment (Spheroids)
Ultra-Low Attachment (ULA) surfaces feature a covalently bound hydrogel layer that effectively inhibits cellular attachment. It forces cells into a suspended states that enables 3D cell culture
Applications
Organoid Model Applications
Organoid Model Applications
Modeling Cancer
It can edit DNA at precise locations faster, more accurate and more efficiently than existing genome editing methods. When combined with organoid technology, it allows for better genetic and drug screening models and opportunities to study organ development.
It can edit DNA at precise locations faster, more accurate and more efficiently than existing genome editing methods. When combined with organoid technology, it allows for better genetic and drug screening models and opportunities to study organ development.
CRISPR
The development of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 genome editing technology enables fast genome engineering.
The development of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 genome editing technology enables fast genome engineering.
Personalized Medicine
Personalized medicine enables targeted treatment for an individual at a molecular and pharmacogenomic level to maximize the effect of a treatment. Organoids, derived from an individual’s stem cells, progenitor cells or from induced pluripotent stem cells, can be used for disease modeling, to test the efficiency and dosage of a drug, and for regenerative medicine.
Personalized medicine enables targeted treatment for an individual at a molecular and pharmacogenomic level to maximize the effect of a treatment. Organoids, derived from an individual’s stem cells, progenitor cells or from induced pluripotent stem cells, can be used for disease modeling, to test the efficiency and dosage of a drug, and for regenerative medicine.
Bioprinting
Bioprinting fabricates a 3D tissue-like construct, layer-by-layer using cells, spheroids, or organoids suspended in a bio-ink. 3D bioprinting has been used for the generation of multilayered skin, bone, liver, and cartilage tissue models in research, toxicology, and drug-screening studies. Bioprinting makes it possible to reproduce structural features seen in vivo and explore the cell-to-cell relationships that affect tissue functionality.
Bioprinting fabricates a 3D tissue-like construct, layer-by-layer using cells, spheroids, or organoids suspended in a bio-ink. 3D bioprinting has been used for the generation of multilayered skin, bone, liver, and cartilage tissue models in research, toxicology, and drug-screening studies. Bioprinting makes it possible to reproduce structural features seen in vivo and explore the cell-to-cell relationships that affect tissue functionality.
Drug Discovery
Drug discovery uses a combination of in vitro and in vivo models, including organoids to identify possible drug candidates. Drug Discovery is a lengthy and expensive procedure that screens millions of compounds for a selective few active ingredients that can be developed into medicine used to treat specific diseases.
Drug discovery uses a combination of in vitro and in vivo models, including organoids to identify possible drug candidates. Drug Discovery is a lengthy and expensive procedure that screens millions of compounds for a selective few active ingredients that can be developed into medicine used to treat specific diseases.
Organs-on-a-Chip
Organ-on-a-chip are microdevices with hollow microfluids channels lined with human organ specific cells interfaced with human endothelial vasculature. This imitates the microarchitecture and function of living human organs and is a human in vivo model for drug testing.
Organ-on-a-chip are microdevices with hollow microfluids channels lined with human organ specific cells interfaced with human endothelial vasculature. This imitates the microarchitecture and function of living human organs and is a human in vivo model for drug testing.
Support
Scientific Support–Setting a New Industry Standard
A Workflow Solution Tailored to Meet your Specific Goals
A Workflow Solution Tailored to Meet your Specific Goals
Contact UsOur scientific teams in the field and in the lab are on standby and ready to help your 3D spheroid model discovery.
Our scientific teams in the field and in the lab are on standby and ready to help your 3D spheroid model discovery.
Scientific Support—Setting A New Industry Standard
Scientific Support—Setting A New Industry Standard
Contact UsOur commitment to enabling your cell culture success doesn’t end with the sale. Our expert scientists continually work to perfect our tools to empower your discovery. And we're here for you at every step of the research process. Need help selecting the right surface? Dealing with complex cells? Looking for custom capabilities? Depend on your partner, Corning, to help resolve your specific challenges.
What you need, when you need it—we're your trusted partner for ensuring you choose the right tools for discovery in your lab.
Our commitment to enabling your cell culture success doesn’t end with the sale. Our expert scientists continually work to perfect our tools to empower your discovery. And we're here for you at every step of the research process. Need help selecting the right surface? Dealing with complex cells? Looking for custom capabilities? Depend on your partner, Corning, to help resolve your specific challenges.
What you need, when you need it—we're your trusted partner for ensuring you choose the right tools for discovery in your lab.
